Expression of Eukaryotic Gene in E. coli

Gene expression is the process by which inheritable information from a gene, such as the DNA sequence, is made into a functional gene product, such as protein or RNA. Several steps in the gene expression process may be modulated, including the transcription step and translation step and the post-translational modification of a protein. A wide range of microorganisms produce invertase and can, thus, utilize sucrose as a nutrient. Commercially, invertase is biosynthesized chiefly by yeast strains of Saccharomyces cerevisiae or Saccharomyces carlsbergensis. The bacteria can also be grown easily and its genetics are comparatively simple and easily-manipulated, making it one of the best-studied prokaryotic model organisms and an important species in biotechnology. E. coli. The objectives of the present study is Isolation and identification of yeast (Saccharomyces cerevisiae) from bakers Yeast. Separation of protein by SDS PAGE,Gene Cloning and Expression of Cloned Gene.